Description
TRI-Color Pre-Stained Protein Ladder High Range (9-245 kDa) BYPL100 is a ready to use three-color
protein standard with 14 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine buffer (9 to 235 kDa in BisTris (MOPS) buffer and 10 to 235 kDa in Bis-Tris(MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, as separated on SDS-PAGE (Tris-Glycine buffer). TRI-Color Pre-Stained Protein Ladder High Range (9-245 kDa), BYPL100 is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or
nitrocellulose), and for approximating protein size.
The ladder is supplied in gel loading buffer and is ready to use. 
Do NOT heat, dilute, or add reducing agents before loading.
 
Features
Ready-to-use Premixed with a loading buffer for direct loading, no need to boil.
Two reference bands 75 kDa (red) and 25 kDa (green)
 
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 
 
Quality Control
Under suggested conditions, TRI-Color Protein Maker resolves 13 major bands in SDS-PAGE (Bis-Tris gel, MES buffer) and after Western blotting to nitrocellulose membrane. 
 
Storage
4°C for 3 months
-20°C for 24 months
 
Tag
BYBYPL700